[Objective] Improve the overall strength of the slope greening substrate, improve its anti-scouring performance, and provide theoretical basis and technical support for the preparation of new ecological substrate and slope vegetation restoration. [Methods] The orthogonal test was carried out to prepare the microbial solidified ecological substrate with the influence factors of cement concentration, plant fiber, compound microbial fertilizer and water retaining agent. The shear strength of the substrate with different proportions was compared, and the micro-mechanism of the strength improvement of the microbial solidified ecological substrate was analyzed by high-definition microscope. A pot experiment was conducted to study the seedling emergence characteristics and vegetation growth of tall fescue and alfalfa in different substrate ratios. Based on the characteristics of shear strength and plant growth, the optimal substrate formula was screened by entropy method. [Results] ( 1 ) The microbial curing has a significant effect on the improvement of the shear strength of the substrate, the cohesion increases by 133.93 % ~ 355.65 %, and the internal friction angle increases by 35.69 % ~ 58.97 %. ( 2 ) The concentration of cementing solution is the most significant factor affecting the emergence time and emergence rate of plants. With the increase of cementing solution concentration, the emergence time is delayed and the emergence rate is significantly reduced. ( 3 ) The growth indexes of the plants in the substrate group were significantly better than those in the control group. The growth and development of tall fescue were the best in the substrate with the cement concentration of 0.2 mol / L and the mass ratio of plant fiber, compound microbial fertilizer and water retaining agent of 1 : 2 : 0.09. Alfalfa had the best growth and development in the substrate with 0.2 mol / L cementing solution concentration, plant fiber, compound microbial fertilizer and water retaining agent mass ratio of 3 : 1 : 0.06. [Conclusion] It is feasible and reliable to apply microbial curing to the preparation of substrate. Microbial curing ecological substrate has good development potential.