[Objective] The inter-provincial border area is a special unit of regional economy in China, and the analysis of its ecosystem service value combined with land use can provide scientific basis for the overall development of eco-economy in the inter-provincial border area. [Methods] Based on the PLUS model and equivalent factor method, the ecosystem service value (ESV) of Huaihai Economic Zone from 2000 to 2020 was evaluated based on the analysis of the evolution characteristics of land use change in Huaihai Economic Zone from 2000 to 2020, and the land use pattern and ecosystem service value (ESV) under different scenarios in 2030 were predicted. [Results]① The transfer between cultivated land and construction land was the closest in the study area from 2000 to 2020. The transfer area of cultivated land was 6127.58km², of which 89.76% was converted to construction land. ② Under the natural development scenario, the area of cultivated land, forest land and grassland decreased, while the area of construction land, water area and unused land increased; Under the economic development scenario, the variation range of local classes is greater than that of natural development scenario, and the ecological environment will be adversely affected. Under the ecological protection scenario, the area of forest land, grassland and water area will increase, and the ecological environment will be significantly improved. Under the overall development scenario, the class area of each region is basically between the economic development and ecological protection scenario. ③ From 2000 to 2020, the ESV of the study area will decrease first and then increase. In 2030, the ESV of natural development, ecological protection and integrated development will increase compared with that of 2020, while it will decrease slightly under the economic development scenario. The spatial distribution characteristics of ESV are basically consistent with the pattern of land use. The high-value area is mainly distributed in the river and lake area of the study area, the median area is mainly dispersed around the high-value area, and the low-value area and low-value area are mainly connected and clustered in the central urban area of each city in the study area. [Conclusion] In the future, Huaihai Economic Zone should pay attention to the rational layout of territorial spatial structure and the protection of ecological environment, improve the quality of ecological system and promote regional sustainable development.