[Objective] The regional ecological environmental effects caused by land use changes in Nanchang City by determining the quantitative relationships between regional land and resource development and ecological environments were analyzed in order to providing support for regional ecological environmental protection, ecological civilization construction, and green development.[Methods] The temporal and spatial change trajectory of land use changes was calculated by using a land use transfer matrix and a nuclear density analysis model. The impact of land use changes on the ecosystem was deduced by an eco-environmental quality index and the ecological contribution rate.[Results] ① From 2000 to 2020, the land use change in Nanchang City was mainly from grassland and cultivated land to construction land, from forest land and water area to cultivated land, and from cultivated land to water area; ② From 2000 to 2020, the spatial agglomeration evolution of land use change in Nanchang City was mainly concentrated in the northwest, and the conversion of cultivated land to water area was the main reason for the improvement of the ecological environment in the study area. The conversion of water area to cultivated land, and cultivated land to construction land were the main reasons for the degradation of the regional ecological environment.[Conclusion] The eco-environmental quality index of Nanchang City in 2000, 2010 and 2020 was 0.354, 0.351, and 0.352. This index was relatively stable, but the eco-environmental quality index in the region showed a downward trend. It will be necessary to strictly control the boundary of urban development and to implement an ecological red-line protection policy in the future.