Abstract:[Objective] The effects of mixed additions of a water-retaining agent and bacterial agents on soil characteristics were determined in order to provide a theoretical basis for promoting mine vegetation restoration.[Methods] Different concentrations of a water-retaining agent and mineral-solubilizing microbial agents were added to the potted soil of Robinia pseudoacacia. The concentrations of the water-retaining agent were:B1 (0%), B2 (0.3%), and B3 (0.5%). The microbial-agent treatments were J0 (without added microorganisms), J1[adding NL11(Bacillus thuringiensis)], J2[adding NL11 + NL11(Streptomyces thermocarboxydus)], J3[adding NL11+NL1+NL15(Gongronella butleri)]. The effects of mixed additions of the water-retaining agent and microorganisms on R. pseudoacacia were determined by analyzing the growth, soil nutrients, soil organic carbon, and soil enzyme activity of R. pseudoacacia.[Results] ① The addition of microbial strains increased soil nutrient content and soil enzyme activity. The J2 microorganism treatment performed the best. ② Readily available soil nutrients initially increased and then decreased with increasing concentration of the water-retaining agent. The greatest effect on soil nutrient and enzyme activity was observed for the 0.3% water-retaining agent concentration. ③ The combinations of J2B1, J3B0, and J2B2 had the greatest effect on increasing soil nutrients and enzyme activities.[Conclusion] The addition of soil microbial agents and a water-retaining agent to the soil increased soil nutrients and enzyme activities. The combinations of J2B1, J3B0, and J2B2 had the greatest effect.