[Objective] The impact of water storage variation on the local ecological environment and economic construction in the Horqin sandy land was studied in order to provide reference for the protection and sustainable utilization of local water resources.[Methods] Using gravity recovery and climate experiment(GRACE) Release-05 data from July 2003 to December 2010 provided by center for space research(CSR), the long-time series of terrestrial water reserves in Horqin sandy land were retrieved by using the filtering method combining the decorrelation filter with the Gauss smoothing filter (smooth radius 300) and the scaling factor method. The result was compared with the Climate Prediction Center(CPC) hydrological model inversion. Based on soil moisture content changes calculated by GLDAS hydrological model, the temporal and spatial variation of ground water storage was retrieved. This result was preliminarily verified by monitoring well data and feed water information.[Results] The reduction rates of terrestrial water storage and ground water storage were 13.2±2.6 and 13.5±1.9 mm/a, respectively from July 2003 to Decomber 2010.[Conclusion] Drought and agricultural irrigation are one of the important reasons leading to the decrease of surface water and groundwater level in Horqin sandy land.