[Objective] The effects of water stress on water consumption and yield of wine grapes were studied in order to provide a scientific basis for water-saving and efficient irrigation of wine grapes in Hexi desert oasis region.[Methods] Two water stress levels of mild (65% field capacity, FC) and moderate (55% FC) were set in the five growth stages of wine grapes (germination, shoot growth, flowering, fruit enlargement and coloring mature stage), respectively, with the lowest limit of 75% FC as a control. The effects of water stress on water consumption intensity, yield and water use efficiency of wine grapes were studied from 2015 to 2016.[Results] ① Water stress at every growth stage of wine grapes significantly affected (reduced) the daily water consumption intensity of grapes, and the effect of water stress in short growth stage (such as germination, shoot growth, flowering stage) could be automatically eliminated after rehydration, while the effect of water stress in long growth period (fruit enlargement and coloring mature stage) was persistent across the year, and it was difficult to recover in a short time after rehydration. ② The yield of mild water stress at germination stage reached the maximum in the two experimental years, it was up to 14 868, 15 306 kg/hm², respectively, and the water use efficiency also reached the highest (2.77, 2.96 kg/m³). The water use efficiency of mild water stress at coloring maturity stage could be improved without no obvious yield reduction. The moderate water stress at shoot growth, flowering and fruit expansion stage could significantly reduce grapes yield, and the reduction rate reached to 39%~49% under water stress at fruit expansion stage.[Conclusion] The growth period and duration of water stress have important effects on water consumption intensity of grapes. Long-term water stress have a continuous effect on water consumption of wine grapes across the year. Mild water stress at germination or coloring maturity stage, and adequate water supply at other growth stages are the efficient irrigation modes for wine grapes in Hexi desert oasis region.