[Objective] Taking Huainan City of Anhui Province as an example, this paper discussed the influence of land use change on the ecosystem service value in the resource-based cities, in order to provide scientific references for the city's ecological, economic, ecological environment protection, the future exploitation and construction of the city, and the development of agriculture as well.[Methods] Based on related studies on land use and the method of ecosystem service value assessment, using GIS technology, this paper analyzed the land use changes of Huainan City from 2000 to 2010 and calculated ecosystem service value to study how ecosystem service value changed with the land use.[Results] The changes of land uses in Huainan City were significant from 2000 to 2010, among which, the areas of cropland, settlement land, industrial and mining land, and water got remarkable changes. Total ecosystem service value of the study area increased from about 1.172 billion yuan in 2000 to about 1.133 billion yuan in 2010, which showed an decreasing tendency with a percentage of 3.33%. The land use structure had changed, of which the cropland and woodland had reduced, settlement land, industrial and mining land, and water area had increased. The sensitivity analysis showed that the ecosystem service value(ESV)lacked of flexibility, indicating that the results of ecosystem service value evaluation are reliable.[Conclusion] Huainan City should attach great importance to the impact of land use change on the value of ecosystem services, optimize the structure of land use, pay more attention to the increase of ecological land, and steadily improve the value of regional ecosystem services.