盐胁迫对蒙古黄芪种子萌发与幼苗生长的影响
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甘肃省科技支撑项目“甘肃省黑果枸杞种质资源调查、收集及评价”(144NKCA045);甘肃农业大学青年研究生指导教师扶持资助项目(GAU-QNDS-201712)


Effect of Salt Stress on Seed Germination and Seedling Growth of Astragalus Mongholicus
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    [目的]分析蒙古黄芪种子在不同浓度NaCl,Na2SO4和NaHCO3处理下的种子萌发和幼苗生长情况,为蒙古黄芪在盐碱地的引种、驯化和种植提供理论依据。[方法]通过发芽盒进行种子萌发出苗试验,测定分析蒙古黄芪种子在不同浓度NaCl,Na2 SO4和NaHCO3处理下的萌发率、发芽势、相对盐害率及其苗根长、苗高及根茎比。[结果]①蒙古黄芪种子在NaCl<0.2%,Na2SO4<0.6%和NaHCO3<0.3%的盐胁迫下能够正常发芽,萌发率和发芽势均高于对照,在大于此浓度的盐胁迫下萌发率和发芽势均开始下降。②蒙古黄芪种子在NaCl<0.6%,Na2SO4<0.8%和NaHCO3<0.3%时的相对盐害率为负值,促进种子萌发,大于此浓度时,相对盐害率为正值,抑制种子萌发。③蒙古黄芪幼苗的茎长、根长及根茎比随着盐浓度的增大而增大,当NaCl和Na2SO4浓度分别在0.4%和0.6%时,黄芪幼苗的茎长和根长达最大,NaHCO3浓度分别在0.6%和0.3%时,黄芪幼苗的茎长和根长达最大,超过此浓度黄芪幼苗的茎长和根长开始减小;当NaCl,Na2 SO4和NaHCO3浓度分别为0.6%,0.8%和0.3%时根茎比达最大,分别为1.658,1.709,1.291,超过此浓度,根茎比开始下降,但均大于1。[结论]蒙古黄芪在NaCl<0.6%,Na2SO4<0.8%和NaHCO3<0.3%的盐胁迫下种子和幼苗能够正常萌发和生长。

    Abstract:

    [Objetive] In order to provide the theoretical basis for Astragalus mongholicus introduction, domestication and planting in saline land, the seed germination and seedling growth of A. mongholicus were investigated in different concentrations of NaCl, Na2SO4 and NaHCO3 treatment to understand the effect salt stress on A. mongholicus.[Methods] Seed germination test were conducted in germination box, and the germination rate, germination potential, relative salt injury rate, root length, seedling height and ratio of root and stem of A. mongholicus were measured in different concentrations of NaCl, Na2SO4 and NaHCO3 treatments.[Results] ① A. mongholicus seed germinated normally under the salt stress with the concentration of NaCl<0.2%, Na2SO4<0.6% and NaHCO3<0.3%, and the germination rate and germination potential were all greater than that of control treatment, vice versa. ② The relative salt damage rate of A. mongholicus seed was negative at low concentration (NaCl 0.6%, Na2SO4 0.8%, NaHCO3 0.3%) as it promoted the seed germination, while it inhibited the germination of A. mongholicus when the concentration was higher. ③ The stem length, root length and ratio of root and stem of A. mongholicus seedling increased with the increase of salt concentration. When the concentration of NaCl and Na2SO4 were 0.4%, 0.6% and 0.6%, 0.3%, respectively, the stem length and root length of A. mongholicus seedling were the maximum. While the stem length and root length of A. mongholicus seedling started to decrease as the concentration of NaCl and Na2 SO4 increased. The maximum ratio of root and stem was 1.658, 1.709 and 1.291, respectively, when the concentrations of NaCl, Na2SO4 and NaHCO3 was 0.6%, 0.8% and 0.3% respectively, while it decreased when the concentrations increased.[Conclusion] The seed and seedling of A. mongholicus could germinate and grow normally under salt stress with the concentrations of NaCl<0.6%, Na2SO4 <0.8% and NaHCO3 <0.3%.

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马彦军,倪强,杨永义,卢瑜,马瑞.盐胁迫对蒙古黄芪种子萌发与幼苗生长的影响[J].水土保持通报,2018,38(3):106-111

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  • 收稿日期:2017-12-14
  • 最后修改日期:2018-01-01
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  • 在线发布日期: 2018-07-06
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