[Objective] The impacts on germination characteristics of soil seed bank(SSB) by adding different substrates were researched to provide valuable reference for the application of soil seed bank in vegetation restoration project in the future.[Methods] In the light of experience from Japan vegetation restoration, greenhouse germination was used to explore the impacts on germination characteristic of SSB by adding different substrates, such as organic substrate(peat), inorganic substrate(perlite) and mixed substrate(peat and perlite).[Results] Seed density and species diversity indexes were apparently improved by adding mixed substrate(peat and perlite). The mixing ratio with topsoil of 20% and 30% had witnessed the highest Shannon-Wiener diversity index and soil seed bank density. For germination curves of SSB, the mixed substrate group showed greater advantage than those of control group and other groups. Multiple regression analysis between soil seed bank density and soil properties, including C, N, P, K elements, showed that soil properties had extremely significant influence on the density of soil seed bank. CCA analysis showed that most plants had a certain degree of requirements on moisture content, C, N, P and K elements.[Conclusion] As a kind of artificial measures, adding mixed substrate to topsoil can promote the seed density and species diversity effectively, which is valuable for the vegetation restoration.