[Objective] The aim of this study is to analyze the effect of Pisolithus tinctorius(PT) inoculant on soil properties and Pinus tabulaeformis seedlings, in order to provide a scientific basis for vegetation restoration in arid and semi-arid Loess Plateau.[Methods] Field experiments were conducted on a newly established 6 a P. tabulaeformis seedlings site at three slope positions(upper, middle, lower) from March to October in 2013 in the Northern Shaanxi loess plateau region. The conventional fertilization was used as control(CK).The effects of PT inoculants on ground diameter, height and growth and survival rate of the 6-year seedlings and soil nutrient contents at different depths (0-60 cm) were investigated.[Results] The average increment of P. tabulaeformis seedlings treated with PT was 4.5 mm, which was 2 times than the control group. The total tree height was 10 cm, which was 54% higher than the control group. The mortality rate was 9% lower than the control group. With the application of PT inoculant, the average organic matter content reached to 7.8 g/kg, which was 2 times higher than the CK. The average total nitrogen content was 0.41 g/kg, and the available potassium was 42.4 mg/kg, which was 52% and 26% higher than that of CK, respectively. There was no significant difference in available phosphorus between PT inoculant treatment and CK. Slope position did not show significant effects on the base diameter and seedling height, but showed remarkable effects on the content of soil organic matter, total nitrogen, and available potassium.[Conclusion] PT inoculant can improve soil properties, increase the survival rate and growth of P. tabulaeformis seedlings. PT inoculant can be used in the P. tabulaeformis seedling afforestation practice in Loess Plateau.