[Objective] Freezing and thawing is one of the major natural phenomena in the black soil area. The effects of freezing and thawing cycling on eroded gully development were studied to prevent freezing and thawing erosion, and then protect the black soil resources. [Methods] The soil physical properties, variation of soil body and gully bank were measured during freezing and thawing process by field measurements and laboratory simulations. [Results] Freezing and thawing cycling increased soil moisture, porosity and frost heaving amount, and the bulk density and the content of soil aggregate were reduced. [Conclusion] Freezing and thawing cycling results in the decrease of soil erosion resistance, it is the main factor leading to an aggravation of erosion gully development.