[Objective] To distinguish potential ecological problems of natural environment in Chengdu City of Sichuan Province, and guide the regional ecological protection and development.[Methods] Based on GIS spatial analysis, we established the sensitivity evaluation index of soil erosion, biodiversity and acid rain.[Results] (1) Main soil erosion in the study area was light erosion, and extremely high erosion was distributed in the western mountain around the basin. (2) The biodiversity and habitat sensitivity in the study area were generally high, especially in the regions of the western and eastern of Chengdu City. Extremely high sensitive areas were distributed in Chaping and Qionglai Mountain areas of Northwest Chengdu City. (3) The acid rain in the study area was generally low sensitive, followed by the moderate sensitive areas. Extremely high sensitive regions were mainly distributed in the areas that was at an elevation of 1 650~3 200 m in the west of Chengdu City with the coniferous, broadleaf mixed forest and original coniferous forest. (4) The eco-environment sensitivity was mainly non-sensitivity, followed by moderate sensitive areas and extremely high sensitive areas. Extremely high sensitive areas were mainly distributed in the northwest and southwest mountain areas around the basin, including the Longxi, Hongkou, Baishuihe national nature reserve.[Conclusion]Highly sensitive and extremely sensitive areas are mainly distributed in the western mountain areas around the basin, these regions are the key areas of regional ecological protection.