Taking the rhizosphere soil of artificial seabuckthorn forest in loess hill and gully region of Eastern Gansu Province as experiment objects, the soil humus composition and combined form and optical properties of humic acid in different recovery stages of artificial seabuckthorn forest were investigated. The results showed that the organic carbon content in humin(HM) >humic acid(HA) >fulvic acid(FA) in different years, meanwhile, PQ(proportion of HA) of CK >the soil of artificial seabuckthorn forest of 7 years old >in 2001 soil of artificial seabuckthorn forest of 10 years >the soil of artificial seabuckthorn forest in 1995, and all the PQ were more than 52% at each layer of the artificial seabuckthorn forest soil of 15 years old. The value of E₄/E₆ and hue coefficient(ΔlgK) demonstrated that:15 years >10 years >CK >7 years; the humification degree of soil of 15 years was significantly higher than that of 10 years, CK and 7 years, which demonstrated that, along with recovery time, humification degree of soil trend to simple, and the oxidation stability of soil humic acid decreased. Meanwhile, it was adversed to the advancement of soil fertility. Therefore, artificial seabuckthorn forests should be regenerated when it was planted more than 15 years to prevent soil erosion and improve soil fertility.