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砒砂岩中植物促生芽孢杆菌的筛选及其对土壤的改良作用
邓琳1, 王涛1, 殷涂童2, 何琳燕2, 杨才千1, 梁止水1
1.东南大学 土木工程学院, 江苏 南京 211189;2.农业部农业环境微生物重点实验室 南京农业大学 生命科学学院, 江苏 南京 210095
摘要:
[目的] 筛选砒砂岩土壤中植物促生芽孢杆菌,为微生物强化植物改良砒砂岩土壤的科学设想提供资源和技术基础。[方法] 以产生植物激素IAA(indole acetic acid)、铁载体和生物膜为筛选指标,从内蒙古砒砂岩区土壤和植物样品中筛选植物促生芽孢杆菌,采用盆栽试验探究植物促生芽孢杆菌改良砒砂岩土壤特性和促进苜蓿和黑麦草生长的作用。[结果] 筛选到的12株芽孢杆菌产生IAA、铁载体和生物膜的能力不同,分别属于Bacillus halotolerans,B.atrophaeus,B.siamensisB.zhangzhouensis种群。与对照相比,B.halotolerans P75能够显著增加砒砂岩土壤的有机质含量(24.7%)、速效磷含量(11.9%)和速效钾含量(21.0%)等养分指标,每1 g土壤可培养细菌达到7.4 lg CFU,土壤蔗糖酶活性显著增强(58.8%)。接种B.halotolerans P75后,在砒砂岩土壤中生长的苜蓿和黑麦草干重增加22.3%~81.5%,[结论] 从生长于砒砂岩土壤的苜蓿根内筛选到B.halotolerans P75,可提高砒砂岩土壤肥力,促进苜蓿和黑麦草生长,具有强化植物改良砒砂岩土壤的潜力。
关键词:  砒砂岩  植物促生细菌  芽孢杆菌  改良  土壤特性
DOI:10.13961/j.cnki.stbctb.2019.05.029
分类号:Q93;S156;S182
基金项目:国家重点研发计划项目“砒砂岩区生态安全保障技术”(2017YFC0504505);国家自然科学基金项目“卤代硝基甲烷在紫外/氯共同消毒过程中生成与降解的特性及机制”(21677032)
Isolation of Plant Growth Promoting Bacillus Spp. and Their Impact on Feldspathic Sandstone Soil Characteristics
Deng Lin1, Wang Tao1, Yin Tutong2, He Linyan2, Yang Caiqian1, Liang Zhishui1
1.School of Civil Engineering, Southeast University, Nanjing, Jiangsu 211189, China;2.Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
Abstract:
[Object] The characteristics of the plant growth promoting Bacillus spp. in a feldspathic sandstone soil were investigated in order to provide resources and technical information for feldspathic sandstone soil improvement.[Methods] Bacillus spp. was isolated from plants and feldspathic sandstone soil in a region of Inner Mongolia. The bacterium were identified by 16S rDNA sequence analysis. The ability of Bacillus spp. for producing indole acetic acid (IAA), siderophores, and biofilms were tested. Greenhouse experiments were conducted to evaluate the effect of Bacillus spp. on the quality of feldspathic sandstone soil, and on the growth of alfalfa (Medicogo sativa) and perennial ryegrass (Lolium perenne).[Results] Twelve strains of Bacillus spp. were screened and had different production of IAA, siderophores, and biofilms, which belong to B. halotolerans, B. atrophaeus, B. siamensis and B. zhangzhouensis. The isolated B. halotolerans P75 was able to significantly increase the content of soil organic matter (24.7%), available phosphorus (11.9%), and available potassium (21.0%) in the sandstone compared with the non-inoculated control. Soil invertase activity (58.8%) and the number of culturable bacteria (7.4 lg CFU in 1 g soil) also significantly increased after B. halotolerans P75 inoculation. Moreover, B. halotolerans P75 significantly increased the dry weight of alfalfa and perennial ryegrass grown on the feldspathic sandstone soil by 22.3%~81.5% in comparison to those of the control.[Conclusion] Our results suggest that the B. halotolerans P75, isolated from the alfalfa roots growing in feldspathic sandstone area, can increase the fertility of feldspathic sandstone soil and promote the growth of alfalfa and ryegrass, and has great potential for the phytoremediation and improvement of feldspathic sandstone soil.
Key words:  feldspathic sandstone  plant growth promoting bacteria  Bacillus  improvement  soil quality