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覆盖和埋置白三叶对黄土高原苹果园土壤细菌多样性的影响
马震珠1, 魏倩倩2, 李尚玮1, 杨文权2, 寇建村1
1.西北农林科技大学 草业与草原学院, 陕西 杨凌 712100;2.西北农林科技大学 生命科学学院, 陕西 杨凌 712100
摘要:
[目的] 探究白三叶在不同降解时期果园土壤细菌多样性的变化,为果园白三叶的利用及果园生草模式的推广提供理论依据。[方法] 在陕西省洛川县苹果园内分别以覆盖和埋置两种方式将白三叶(Trifolium repens)返园,通过高通量测序研究两种返园方式下白三叶降解1,3,6,12个月时土壤细菌的多样性、丰度及群落结构。[结果] ①白三叶返园处理下,土壤微生物优势菌群种类未表现出显著变化,丰度存在差异,返园处理组土壤中出现梭杆菌门(Acidobacteria)、广古菌门(Euryarchaeota)、泉古菌门(Crenarchaeota)、螺旋体门(Spirochaetes)和硝化螺旋菌(Nitrospira)门等特有菌群。②白三叶返园对苹果园土壤细菌群落分布存在显著影响(p<0.05),且不同降解时期存在差异。两种返园处理组中酸杆菌门(Acidobacteria)、拟杆菌门(Bacteroidetes)、变形菌门(Proteobacteria)丰度增高,厚壁菌门(Firmicutes)丰度降低。③随着白三叶降解时间增加,芽单胞菌(Gemmatimonas)、黄杆菌(Flavobacterium)、OpitutuArenimonas属的细菌相对丰度增高,硝化螺旋菌属(Nitrospira)、奈瑟菌属(Neisseria)、PirellulaSteroidobacter的丰度降低。[结论] 覆盖和埋置白三叶均能改善土壤菌群结构,提高有益菌丰度,促进土壤微生物生态系统物质循环。
关键词:  白三叶  苹果园  覆盖  埋置  细菌多样性
DOI:10.13961/j.cnki.stbctb.2019.05.020
分类号:S154.3;S182
基金项目:国家自然科学基金项目“黄土高原水热季节耦合对苹果园白三叶降解形成土壤有机质的影响机理”(31302027)
Effects of Covering or Burying Trifolium Repens on Diversity of Soil Bacteria Community in an Apple Orchard in Loess Plateau
Ma Zhenzhu1, Wei Qianqian2, Li Shangwei1, Yang Wenquan2, Kou Jiancun1
1.College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China;2.College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China
Abstract:
[Objective] The return of Trifolium repens to an apple orchard was investigated to understand the effects on soil bacteria diversity in different degradation stages, and to provide theoretical basis for the utilization of T. repens in orchards and the promotion of pasture-planting in orchards.[Methods] Fresh T. repens was covered or buried in an apple orchard in Luochuan County, Shannxi Province. At 1, 3, 6 and 12 months of T. repens degradation, soil samples were collected, and high-throughput sequencing technology was performed to determine the diversity, richness and structure of soil bacteria.[Results] ① There were no significant differences in the diversity of preponderant bacteria after T. repens returning, but some richness was different. Acidobacteria, Euryarchaeota, Crenarchaeota, Spirochaetes and Nitrospira were found in the soil of the return treatment group. ② There were some remarkable differences in community distribution of soil bacteria (p<0.05), varying in different degrading period after the return of T. repens. The richness of Acidobacteria, Bacteroidetes and Proteobacteria increased, but that of Firmicutes decreased in soil samples from the two treatment groups compared with the control. ③ Over time, the richness of Gemmatimonas, Flavobacterium, Opitutu, and Arenimonas increased in groups in which T. repens had returned; however, the richness of Nitrospira, Neisseria, Pirellula, and Steroidobacter decreased.[Conclusion] Either covering or burying T. repens could optimize structure of soil bacteria community, increase probiotics abundance and improve the matter cycle in the microbial ecosystem.
Key words:  Trifolium repens  apple orchard  covering  burying  bacteria diversity