[Objective] Studying the spatiotemporal variation characteristics of NDVI in Jiangsu Province and the relationships between climatic factors with NDVI, in order to provide conferences for planing policies and countimeasures for eco-environment protection.[Methods] Linear regression trend method was used to analyze the spatiotemporal variation characteristics of NDVI, and correlation analysis method was used to analyze the relationship between climatic factors with NDVI.[Results] (1) The rising rate of average annual NDVI in Jiangsu Province from 2001 to 2010 was 0.005/a. (2) The average of NDVI in some hilly area were over 0.8, in Yangtze-Huai Plain and the Huang-Huai Plain, were mostly between 0.7 and 0.8.(3) The correlation between annual NDVI and relative humidity was -0.720, the correlation between monthly NDVI and the air temperature in the same month was 0.860; There existed lagging effect and accumulation effect for the influence of rainfall and relative humidity on NDVI, and the lag phase were both a month.[Conclusion] The average annual NDVI in Jiangsu Province had fluctuated uprising tendency from 2001 to 2010. The distribution and the change trend of average NDVI were distinct in different districts. The correlation of relative humidity and NDVI was the highest on the inter annual timescale, and the air temperature had the most important influence on monthly changes of NDVI.