%0 Journal Article %T 花后干旱胁迫对不同持绿型玉米叶片衰老的影响 %T Effect of Different Stay-green Maize on Leaves Senescence After Flowering Under Drought Stress %A 王芳 %A 彭云玲 %A 方永丰 %A 慕平 %A 王威 %A WANG,Fang %A PENG,Yunling %A FANG,Yongfeng %A MU,Ping %A WANG,Wei %J 水土保持通报 %J 水土保持通报英文版 %@ 1000-288X %V 38 %N 4 %D 2018 %P 60-66 %K 玉米;持绿型;干旱胁迫;叶片衰老 %K maize;stay-green;drought stress;leaf senescence %X [目的]探讨不同持绿型玉米抗旱的生理机制差异,为促进作物持绿性状的应用,指导作物抗旱改良工作提供理论依据。[方法]利用盆栽试验,以正常灌水为对照,对持绿型玉米自交系齐319和早衰型玉米自交系B73在开花后进行1周的干旱胁迫处理,测定与抗旱相关的生理生化指标及叶片衰老特性。[结果]在干旱胁迫下,与早衰型玉米B73相比,持绿型玉米齐319叶片POD和SOD活性均较高。齐319叶片过氧化物酶(POD)和超氧化物歧化酶(SOD)活性较对照分别增加了32.53%和18.84%,B73较对照分别增加了12.79%和10.82%。干旱胁迫使两玉米自交系的叶片丙二醛(MDA)含量较对照都有显著增加,齐319较对照增加了14.23%,B73较对照增加了37.43%。与对照相比,齐319叶片脯氨酸含量增加了57.95%,B73叶片脯氨酸含量增加了43.67%。且在干旱胁迫下,持绿型玉米齐319具有相对较高的净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr),绿叶面积、叶片保绿度、光合色素含量均高于早衰型玉米B73,而蛋白水解酶活性以及叶绿素水解酶活性均低于早衰型玉米B73。[结论]持绿型玉米齐319在干旱胁迫条件下表现出较强的抗旱性。 %X [Objective] The paper aimed to explore the difference of physiological mechanism of different stay-green maizes under drought resistance, which can provide a theoretical basis for promoting the application of crop stay-green traits and guiding the improvement work of crop drought resistance.[Methods] Pot experiment was conducted to investigate the physiological and biochemical indexes related to drought resistance and leaf senescence characteristics of stay green maize inbred lines Qi319 and early senescence maize inbred lines B73 after 1 week of flowering. And the normal irrigation water was made as control.[Results] Under drought stress, the activities of peroxidase(POD) and superoxide dismutase(SOD) of green maize Qi319 were higher than those of early aging maize B73, and Qi319. The two indices of Qi319 increased by 32.53% and 18.84%, B73 increased 12.79% and 10.82% as compared with the control respectively. The leaf MDA contents of the two maizes inbred lines increased significantly compared with the control by drought stress. The MDA content of Qi319 increased by 14.23% while that of B73 increased by 37.43%. Compared with the control, the proline content in the leaves of Qi319 increased by 57.95%, and that of B73 increased by 43.67%. Under drought stress, Qi319 had a relatively high net photosynthesis photosynthetic rate(Pn), stomatal conductance(Gs) and transpiration rate(Tr). The number of green leaves, green leaf area, leaf green retention and photosynthetic pigment content of stay-green maize Qi319 were higher than those of early senescence maize B73, while the activities of protease and chlorophyll hydrolase of Qi319 were all lower than those of early senescent maize.[Conclusion] Under drought stress the stay-green maize Qi319 had strong drought resistance. %R 10.13961/j.cnki.stbctb.2018.04.010 %U http://stbctb.alljournal.com.cn/stbctb/home %1 JIS Version 3.0.0